Last edited by Fenrigal
Friday, August 7, 2020 | History

4 edition of Limiting dilution analysis of cells of the immune system found in the catalog.

Limiting dilution analysis of cells of the immune system

by Ivan Lefkovits

  • 97 Want to read
  • 20 Currently reading

Published by Oxford University Press in Oxford [England], New York .
Written in English

    Subjects:
  • Lymphocytes -- Research -- Methodology.,
  • Plaque assay technique.,
  • Immunology -- Statistical methods.,
  • Dilution.,
  • B-Lymphocytes -- chemistry.,
  • Indicator Dilution Techniques.,
  • B-Lymphocytes -- immunology.,
  • T-Lymphocytes -- chemistry.,
  • T-Lymphocytes -- immunology.,
  • Immunity, Cellular.

  • Edition Notes

    StatementIvan Lefkovits, Hermann Waldmann ; with LDA software developed by P. Rovensky, J. Rubes, and T. Beran.
    ContributionsWaldmann, Herman.
    Classifications
    LC ClassificationsQR185.8.L9 L43 1999
    The Physical Object
    Paginationxvi, 285 p. :
    Number of Pages285
    ID Numbers
    Open LibraryOL387216M
    ISBN 100198501285
    LC Control Number98050046

    Blood analysis, laboratory examination of a sample of blood used to obtain information about its physical and chemical properties. Blood analysis is commonly carried out on a sample of blood drawn from the vein of the arm, the finger, or the earlobe; in some cases, the blood cells of the bone marrow may also be examined. Hundreds of hematological tests and procedures have been developed, and. The immune system is a complex system that is responsible for protecting us against infections and foreign substances. There are three lines of defense: the first is to keep invaders out (through skin, mucus membranes, etc), Organs, Tissues and Cells of the Immune System The immune system consists of a network of lymphatic organs, tissues.

      Limiting dilution analyses. Like those performed in the mouse, limiting dilution analyses provide the ideal system for comparing HSC function between 2 experimental groups. Here, we aimed to generate a similar assay in the zebrafish that would allow quantification of repopulating cell number per marrow volume. The use of compromised marrow cells is common in murine limiting dilution experiments (reviewed by Purton and Scadden). 2 Immunologic matching would be critically important for these WKM carrier cells, as is true in murine experiments. Approximately 10%% of the WKM preparation contains lymphocytes, which if immunologically mismatched would.

      Cells of the immune system. The cells that serve specialized roles in innate and adaptive immune responses are phagocytes, dendritic cells, antigen-specific lymphocytes, and various other leukocytes that function to eliminate antigens. Although most of these cells are found in the blood, their responses to microbes usually occur in lymphoid and.   Such cells have been described both in vitro in the embryonic stem cell (ESC) culture system 8,9 and retrospectively in with limiting cell dilution 23 regulator of the immune system.


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Limiting dilution analysis of cells of the immune system by Ivan Lefkovits Download PDF EPUB FB2

Immunology Today, vol. 5, No. 10, Limiting dilution analysis of cells of the immune system II: What can be learnt. Herman Waldmann and Ivan Lefkovits* Last month (p. ) Herman Waldmann and Ivan Lefkovits described the principles underlying limiting dilution by: Get this from a library.

Limiting dilution analysis of cells in the immune system. [Ivan Lefkovits; Herman Waldmann] -- Piano teacher Kanako, trying to cope with the death of her beloved Pomeranian, considers bringing a new dog into her life. Limiting dilution analysis of cells of the immune system.

Oxford [England] ; New York: Oxford University Press, (OCoLC) Material Type: Internet resource: Document Type: Book, Internet Resource: All Authors / Contributors: Ivan Lefkovits; Herman Waldmann. Full text Full text is available as a scanned copy of the original print version.

Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by page. Last month (p.

) Herman Waldmann and Ivan Lefkovits described the principles underlying limiting dilution analysis. In this article they review some experimental approaches which have been useful in resolving some of the complexities of lymphocyte by:   Immunology Today, voL 5, No.

9, Limiting dilution analysis of the cells of immune system I. The clonal basis of the immune response Ivan Letkovits* and Herman Waldmann Much of what we call 'cellular immunology' depends upon analysis of an immune response brought about by acrobatic manipulations of cells in culture vessels or irradiated mice.

Limiting dilution analysis is a common technique that is used in immunology to estimate accurately the frequency of cells possessing a wide variety of functional activities such as growth.

Keywords: extrapulmonary tuberculosis, limiting dilution analysis, proliferation kinetics, suppression, V-shape LDA INTRODUCTION TB is an increasing problem world-wide, with developed countries experiencing a resurgence of TB which correlates with socio-economic conditions and HIV infection [ 1.

These theoretical models have been expanded by Corley et al. () who used limiting dilution analysis to detect cells mediating negative allogeneic effects to B cells.

It was important to include a reading for zero cells per well, as not all wells may. The book then looks into the analysis of T cell clones and hybridomas using techniques of somatic cell genetics. The clonal analysis by limiting dilution, the characteristics of murine T cell clones reactive with alloantigens and soluble antigens, and the human T cell clones are described as well.

Haematopoietic stem cells (HSCs) are widely studied by HSC transplantation into immune- and blood-cell-depleted recipients. Single HSCs can rebuild the system. A proportion of cells in the peripheral blood were also observed to respond to IL-2 only.

The frequency of cells observed in limiting dilution analysis for PPD and MLS reflected the relative amounts of proliferation to these two antigens in bulk culture lymphocyte transformation tests. Comparative results obtained with slide and optimized modified tray assay methods for limit dilution assay To test the modified tray system for routine application to limiting dilution analysis, low numbers of CBA spleen cells were cultured with thymus filler cells and FLU-POL in flat-bottomed microculture trays as described in Materials and.

Abstract. When T-responder cells are sensitized in vitro to foreign antigen presented on syngeneic cells or towards allogeneic stimulator cells, a proliferative response is initiated in which antigen specific cytotoxic T lymphocytes (CTL) are generated. The induction of CTL, however, requires the collaboration between functionally distinct T cell subpopulations 1–5 and accessory cells from.

A limiting-dilution system is described that makes use of T cell growth factor T cell expansion and allows the determination of precursor frequencies for various regulatory and effector T cells in. (3–5) This approach has the advantage of providing a complete description of the immune system with regard to the specific clones expressed within a B-cell subset.

In addition, as the frequency of a given paratope or idiotope is determined in the absence of antigen, this analysis is preferable to others since it does not depend on a. Taswell C. Limiting dilution assays for the determination of immunocompetent cell frequencies.

Data analysis. J Immunol. Apr; (4)– Mahanonda R, Seymour GJ, Powell LW, Good MF, Halliday JW. Limit dilution analysis of peripheral blood T lymphocytes specific to periodontopathic bacteria. Clin Exp Immunol. Lefkovitz, I., and Waldmann, H.,Limiting dilution analysis of cells in the immune system, Cambridge University Press, Boston.

Google Scholar Marchal, G., Milon, G., and Hurtrel, B.,High radiosensitivity of induction in contrast to expression of cells mediating delayed-type hypersensitivity during response to sheep red blood cells.

Tumor Antigen Tumor Cell Vaccine 51Cr Release Assay Limit Dilution Analysis Leukocyte Depletion These keywords were added by machine and not by the authors.

This process is experimental and the keywords may be updated as the learning algorithm improves. Abstract. Alterations of the immune system during human aging affect different lymphocyte functions.

A progressive modification in the distribution of lymphocyte subpopulations in peripheral blood has been described, the major finding being a decrease in circulating T cells. The immune system in a broad sense is a mechanism that allows a living organism to discriminate between "self" and "non-self." Examples of immune systems occur in multicellular organisms as simple.Priming of virus-immune memory T cells in newborn mice Article (PDF Available) in Infection and Immunity 43(1) February with 9 Reads How we measure 'reads'.In the past, cell clones were typically isolated by basic manual methods, the simplest of these being limiting dilution cloning, in which cells are serially diluted in multiwell plates to a theoretical concentration of less than one cell per well.

After incubation, cells from wells at the lowest dilution showing growth are expanded and screened.